Coordinating Humanitarian Entry in the United States and Mexico: A Bilateral Approach to U.S. Legal Migration

Mexico and the United States have stated a joint interest in reducing illegal immigration through Mexico to the U.S.-Mexican border. Both countries are signatories of the Los Angeles Declaration on Migration and Protection, which pledges a coordinated multilateral approach to addressing migration, and Mexico has worked with the United States on its enforcement efforts, accepting returns from the United States. One untapped area of potential coordination is in each nation's authorization for migrants to temporarily enter their countries for humanitarian reasons.Unfortunately, the lack of coordination has meant that many migrants travel through Mexico and congregate in northern Mexico near the U.S.-Mexican border to try to obtain humanitarian entry into the United States. A better approach would be for Mexico to issue cards for visitors for humanitarian reasons at the Guatemalan??Mexican border, allowing migrants to travel to Mexico City, where they could apply for U.S. parole and fly directly to the United States legally

School-based Smoking Prevention with Media Literacy: A Pilot Study

School-based tobacco prevention programs have had limited success reducing smoking rates in the long term. Media literacy programs offer an innovative vehicle for delivery of potentially more efficacious anti-tobacco education. However, these programs have been neither widely implemented nor well evaluated. We conducted a pre-post evaluation of a cross-disciplinary tobacco media literacy program. The sample consisted of 204 students across six schools. Results indicated that students’ smoking-specific media literacy and general media literacy measures increased significantly over the course of the intervention

An Agenda for Open Science in Communication

In the last 10 years, many canonical findings in the social sciences appear unreliable. This so-called “replication crisis” has spurred calls for open science practices, which aim to increase the reproducibility, replicability, and generalizability of findings. Communication research is subject to many of the same challenges that have caused low replicability in other fields. As a result, we propose an agenda for adopting open science practices in Communication, which includes the following seven suggestions: (1) publish materials, data, and code; (2) preregister studies and submit registered reports; (3) conduct replications; (4) collaborate; (5) foster open science skills; (6) implement Transparency and Openness Promotion Guidelines; and (7) incentivize open science practices. Although in our agenda we focus mostly on quantitative research, we also reflect on open science practices relevant to qualitative research. We conclude by discussing potential objections and concerns associated with open science practices

Activating PIK3CD mutations impair human cytotoxic lymphocyte differentiation and function and EBV immunity

Background Germline gain-of function (GOF) mutations in PIK3CD, encoding the catalytic p110δ subunit of phosphoinositide 3-kinase (PI3K), result in hyperactivation of the PI3K–AKT–mechanistic target of rapamycin pathway and underlie a novel inborn error of immunity. Affected subjects exhibit perturbed humoral and cellular immunity, manifesting as recurrent infections, autoimmunity, hepatosplenomegaly, uncontrolled EBV and/or cytomegalovirus infection, and increased incidence of B-cell lymphoproliferation, lymphoma, or both. Mechanisms underlying disease pathogenesis remain unknown. Objective Understanding the cellular and molecular mechanisms underpinning inefficient surveillance of EBV-infected B cells is required to understand disease in patients with PIK3CD GOF mutations, identify key molecules required for cell-mediated immunity against EBV, and develop immunotherapeutic interventions for the treatment of this and other EBV-opathies. Methods We studied the consequences of PIK3CD GOF mutations on the generation, differentiation, and function of CD8+ T cells and natural killer (NK) cells, which are implicated in host defense against infection with herpesviruses, including EBV. Results PIK3CD GOF total and EBV-specific CD8+ T cells were skewed toward an effector phenotype, with exaggerated expression of markers associated with premature immunosenescence/exhaustion and increased susceptibility to reactivation-induced cell death. These findings were recapitulated in a novel mouse model of PI3K GOF mutations. NK cells in patients with PIK3CD GOF mutations also exhibited perturbed expression of differentiation-associated molecules. Both CD8+ T and NK cells had reduced capacity to kill EBV-infected B cells. PIK3CD GOF B cells had increased expression of CD48, programmed death ligand 1/2, and CD70. Conclusions PIK3CD GOF mutations aberrantly induce exhaustion, senescence, or both and impair cytotoxicity of CD8+ T and NK cells. These defects might contribute to clinical features of affected subjects, such as impaired immunity to herpesviruses and tumor surveillance

A Transgenic Drosophila Model Demonstrates That the Helicobacter pylori CagA Protein Functions as a Eukaryotic Gab Adaptor

Infection with the human gastric pathogen Helicobacter pylori is associated with a spectrum of diseases including gastritis, peptic ulcers, gastric adenocarcinoma, and gastric mucosa–associated lymphoid tissue lymphoma. The cytotoxin-associated gene A (CagA) protein of H. pylori, which is translocated into host cells via a type IV secretion system, is a major risk factor for disease development. Experiments in gastric tissue culture cells have shown that once translocated, CagA activates the phosphatase SHP-2, which is a component of receptor tyrosine kinase (RTK) pathways whose over-activation is associated with cancer formation. Based on CagA's ability to activate SHP-2, it has been proposed that CagA functions as a prokaryotic mimic of the eukaryotic Grb2-associated binder (Gab) adaptor protein, which normally activates SHP-2. We have developed a transgenic Drosophila model to test this hypothesis by investigating whether CagA can function in a well-characterized Gab-dependent process: the specification of photoreceptors cells in the Drosophila eye. We demonstrate that CagA expression is sufficient to rescue photoreceptor development in the absence of the Drosophila Gab homologue, Daughter of Sevenless (DOS). Furthermore, CagA's ability to promote photoreceptor development requires the SHP-2 phosphatase Corkscrew (CSW). These results provide the first demonstration that CagA functions as a Gab protein within the tissue of an organism and provide insight into CagA's oncogenic potential. Since many translocated bacterial proteins target highly conserved eukaryotic cellular processes, such as the RTK signaling pathway, the transgenic Drosophila model should be of general use for testing the in vivo function of bacterial effector proteins and for identifying the host genes through which they function

Early programming of the oocyte epigenome temporally controls late prophase I transcription and chromatin remodelling

Oocytes are arrested for long periods of time in the prophase of the first meiotic division (prophase I). As chromosome condensation poses significant constraints to gene expression, the mechanisms regulating transcriptional activity in the prophase I-arrested oocyte are still not entirely understood. We hypothesized that gene expression during the prophase I arrest is primarily epigenetically regulated. Here we comprehensively define the Drosophila female germ line epigenome throughout oogenesis and show that the oocyte has a unique, dynamic and remarkably diversified epigenome characterized by the presence of both euchromatic and heterochromatic marks. We observed that the perturbation of the oocyte's epigenome in early oogenesis, through depletion of the dKDM5 histone demethylase, results in the temporal deregulation of meiotic transcription and affects female fertility. Taken together, our results indicate that the early programming of the oocyte epigenome primes meiotic chromatin for subsequent functions in late prophase I

Measurement of the inclusive energy spectrum in the very forward direction in proton-proton collisions at root s=13 TeV

Peer reviewe

Haitians Assimilate Well in the United States

Haitian immigrants have received more attention in recent weeks after the U.S. government trapped them in terrible conditions at a camp along the U.S.-Mexico border and is now using extraordinary legal methods to expel more than 500 per day. But there is no reason to oppose Haitian immigration. Despite extreme challenges, Haitians integrate well into the U.S. economy and society. Across numerous economic and social measures, Haitians and their U.S. descendants contribute to and prosper in their new country

Streamlining to End Immigration Backlogs

America's legal immigration system is inefficient; the multidepartmental division of authority, duplicative reviews, antiquated technology use, outdated bureaucratic procedures, unresponsive customer service, intense and unwarranted skepticism applied to all applicants, and lack of accountability or oversight have no parallel in the federal government. These deficiencies have spawned spiraling backlogs, unimaginable wait times, lawsuits by applicants, and countless mistakes—all of which cost people time, money, and the rights to live, work, and join their families.This paper will focus on agencies other than the Department of Justice, which almost exclusively handles removal proceedings in immigration courts. The other agencies have a more significant role in legal immigration procedures.The Department of Homeland Security's U.S. Citizenship and Immigration Services (USCIS) handles the broadest range of requests: petitions by family or employer sponsors, work authorizations, adjustments of status inside the United States, and so on.The Department of State's Bureau of Consular Affairs ("State") processes applications for immigrant (or permanent) and nonimmigrant (or temporary) visas, both of which authorize travel to the United States.The Department of Labor (DOL) oversees wage and employment rules for most temporary and permanent visa programs